BD FACSDiscover A8 Cell Analyser with BD CellView Image Technology

DTU will like to buy a BD FACSDiscover A8 5 Laser 78 detectors from BD Biosciences.
The instrument will include the following :
• Forward Scatter: Label-free brightfield imaging channel that measures light scattered at small forward angles by the cell as it passes through the laser interrogation point. • SSC Imaging: Label-free imaging channel measuring light scattered at 90° perpendicular to the laser path, capturing internal cellular complexity.
• Lightloss Imaging: Measures the total photons lost from the laser beam due to both scattering and absorption by the cell, allowing Label-free brightfield imaging morphological assessment.
• Channel 1: Fluorescence imaging (530/30nm range)
• Channel 2: Fluorescence imaging (600/60nm range)
• Channel 3: Fluorescence imaging (788/225nm range)

These imaging channels enable spatial analysis of protein localisation, cellular interactions, and morphological characteristics that remain invisible to traditional flow cytometry. The system generates quantitative image parameters including:
• Area and Perimeter measurements for size discrimination
• Aspect Ratio and Eccentricity for shape analysis
• Diffusivity for texture characterisation
• Correlation and Distance for multi-channel co-localisation
• Radial Moment for signal distribution analysis.

Applications enabled by this technology include:
• Identification of subcellular protein localisation (nuclear vs cytoplasmic)
• Detection of cell-cell interactions and immunological synapses
• Discrimination of budding yeast cells and cell aggregates
• Analysis of phagocytosis and particle uptake
• Label-free morphological study for downstream sorting.

Complementing the imaging capabilities, the instrument should hold a technology for full-spectrum flow cytometry. This system features:
• 5 laser configuration: 349 nm (UV), 405 nm (Violet), 488 nm (Blue), 561 nm (Yellow-Green), 637 nm (Red)
• 78 fluorescence detectors capturing complete emission spectra
• 8 scatter and imaging detectors
• System-aware spectral unmixing algorithms that adapt to instrument performance in real-time
• Capability for 40+ parameter experiments with optimal resolution and sensitivity

Complementing the imaging capabilities, the instruments should include a technology for full-spectrum flow cytometry. This system features:
• 5 laser configuration: 349 nm (UV), 405 nm (Violet), 488 nm (Blue), 561 nm (Yellow-Green), 637 nm (Red)
• 78 fluorescence detectors capturing complete emission spectra
• 8 scatter and imaging detectors
• System-aware spectral unmixing algorithms that adapt to instrument performance in real-time
• Capability for 40+ parameter experiments with optimal resolution and sensitivity